FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357092
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36480.2688100733037996No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9550.038.3077771
TGGTATC9750.035.8147242
GTATCAA47850.028.9725841
GGTATCA22300.026.1498761
TATCAAC54100.026.1233222
ATCAACG56050.025.1589363
TCAACGC56350.025.0231484
CAACGCA56950.024.9052265
AGAGTAC51200.024.8131911
CAGAGTA52500.024.4055810
AACGCAG59050.023.9264186
ACGCAGA61300.023.0482067
CGCAGAG60750.023.030648
AGTACGG56600.021.91385313
CCGAATT3550.021.69143514
GCAGAGT64950.021.5876589
CGAATTA3500.021.2155515
GAGTACG59200.021.18135312
TGCCGAC4750.020.84719355
TACGGGG22150.020.36238915
Produced by FastQC (version 0.11.5)