FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146812
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31510.2747616871815084No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28130.24528867852795402No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19640.17125736389225085No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT14450.12600147190646768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC15000.056.6656762
GTGGTAT17350.050.2284051
GTATCAA54500.039.7023541
GGTATCA27150.035.0730171
TATCAAC63000.034.6065372
ATCAACG67000.032.4770473
TCAACGC67050.032.4528274
CAACGCA68550.031.6807045
AACGCAG69800.031.1133526
ACGCAGA77850.027.896117
CGCAGAG79100.027.4552738
GCAGAGT83750.026.1350149
GTCGAGG9350.024.5450258
TCGAGGT9750.022.667269
CAGAGTA46600.022.39109610-11
ATCGACC1000.003155975621.249634
AGAGTAC44600.021.0127810-11
GAGTACG51400.019.92843212-13
AGTCGAG14000.019.7317987
GACGTGT1100.005479897419.3178446
Produced by FastQC (version 0.11.5)