FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146812
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22570.19680645127536164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC16350.039.847312
GTGGTAT17450.037.6603621
GTATCAA67050.026.5330721
AGAGTAC47000.025.10361311
GGTATCA34100.024.916451
CAGAGTA47700.024.73521610
TATCAAC73850.023.934752
ATCAACG79550.022.0095333
TCAACGC79650.021.8783764
AGTACGG53300.021.72359513
CAACGCA80600.021.5523015
TACGGGT5250.021.4783715
GAGTACG55000.021.25215712
AACGCAG82250.021.1886276
TACGGGG22400.019.0309515
ACGCAGA92100.018.923357
CGCAGAG92500.018.8448078
GTACGGG60850.018.80223714
GCAGAGT96500.018.209319
ACGGGTT1403.3175875E-617.68036716
Produced by FastQC (version 0.11.5)