FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2674345
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54600.20416214063630533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT25250.034.6305271
TGGTATC24900.034.4386062
CGGGATA2050.029.51559817
GTATCAA90200.026.70491
AGAGTAC57550.024.65874111
TATCAAC100300.023.7305222
TCAACGC99100.023.5728034
CAGAGTA61800.023.45247510
ATCAACG101250.023.2079643
CAACGCA101700.023.0512265
GGTATCA46600.022.2458421
AACGCAG105900.022.2416526
AGTACGG63500.022.00212513
GAGTACG65450.021.59872412
GTACGGG69150.019.92637614
ACGCAGA118450.019.8862237
CGCAGAG118800.019.833948
TACGGGG32100.019.53496415
CCGATCG1008.9793466E-519.25402346
GCAGAGT124000.019.1388139
Produced by FastQC (version 0.11.5)