FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902692
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23610.26155100521551095No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20080.22244575115321724No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13240.14667239767273887No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT11870.13149557102533313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC15800.048.684582
GTGGTAT17750.042.3972171
GTATCAA52800.036.3971181
TATCAAC61200.031.943052
AACGCAG63450.030.743346
TCAACGC63400.030.7005524
GTCGAGG7100.030.5285288
GGTATCA30600.030.1509341
ATCAACG65200.029.9181733
CAACGCA65150.029.8759025
CGCAGAG72550.026.8300868
ACGCAGA73100.026.686367
ATACGGG1001.0528113E-425.5101931
GCAGAGT79750.024.6223419
TCGAGGT9050.022.0733059
GCTTGTA4850.021.9159741
CAGAGTA49500.021.63901510-11
GTATTAT3450.020.95041
ATAGCGG1306.2285614E-419.6156167
AGAGTAC47150.019.47216410-11
Produced by FastQC (version 0.11.5)