FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902692
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11370.12595658319781278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC20850.037.9658282
GTGGTAT21950.035.8306431
GCGTAAC705.6987556E-627.50396552
GTATCAA62900.024.3516041
GCAGTCG600.002033487722.9199713
GGTATCA35800.022.7243293
GCTTGTA3050.022.5404831
AGAGTAC46400.022.28769711
CGGGGCC752.8691962E-422.0031717
TGAACGG752.8724255E-421.99951255
CAGAGTA47650.021.87497310
TATCAAC70900.021.5543542
CGTCCCG804.4552263E-420.6154026
AACGCAG76600.020.0591986
TCAACGC77200.019.829894
CAGTCGG700.004971073519.64568914
ATCAACG78000.019.591273
CAACGCA78550.019.4890825
CGGGGCG856.6758954E-419.41456217
TCGGGTA1352.3410503E-618.33699425
Produced by FastQC (version 0.11.5)