FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402286
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT64200.45782386759904903No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48620.34671957075803367No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT33150.23639970733502297No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32760.23361853430755208No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC16500.059.5062642
GTGGTAT16850.058.5370981
GTATCAA62500.039.385981
GGTATCA33100.037.505761
TATCAAC69100.035.6151852
TCAACGC73350.033.6638834
ATCAACG73900.033.4708483
CAACGCA74850.033.1028145
AACGCAG78350.031.6783126
CGTTAGA703.9354843E-430.367921
ACGCAGA85150.029.1453937
CGCAGAG86250.028.6751468
GCAGAGT89900.027.5109169
CAGAGTA51000.025.20559310-11
GACAGGT6050.021.7845921
GAGTACG58450.021.73844112-13
AGAGTAC49100.021.7237110-11
GTACGGG63100.020.03617714-15
CTATCGG858.26597E-619.99782612-13
AGTACGG56400.018.87427912-13
Produced by FastQC (version 0.11.5)