FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402286
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70600.5034636301011349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC10150.040.36272
GTGGTAT11000.038.0207671
GTATCAA54500.028.5247541
GGTATCA25350.028.3290821
TATCAAC61400.025.6593882
ATCAACG64200.024.2344423
TCAACGC64150.024.0373634
CAACGCA65450.023.6850665
AGAGTAC60850.023.67806611
CAGAGTA63000.023.04211810
AACGCAG67350.023.0168916
ACGCAGA68100.022.8844817
CGCAGAG68750.022.4698268
AGTACGG65950.021.39296713
GCAGAGT72950.021.2553069
ATCCGTT650.003238554321.15558832
TACGGGC9900.021.1151115
GAGTACG68800.020.62449312
CGAGCGT856.6763896E-419.41544216
ACGGGAT11550.019.28936816
Produced by FastQC (version 0.11.5)