FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901622
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46830.24626345298907984No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45500.23926942368146772No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29330.15423675157313071No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT23790.1251037272391674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC23750.053.6841962
GTGGTAT25850.049.342461
GTATCAA87500.039.0182381
GGTATCA47850.034.6531561
TATCAAC99100.034.52322
TCAACGC103150.033.1265034
ATCAACG103450.033.1126063
CAACGCA106600.032.0543985
AACGCAG110550.030.8698256
CGCAGAG122100.027.91498
GTCGAGG14500.027.8440888
ACGCAGA123700.027.622557
GCAGAGT128850.026.5514879
CAGAGTA72300.023.45373310-11
GACAGGT10800.022.8330731
TCGAGGT16650.022.4618669
AGTCGAG22400.021.4391617
AGAGTAC69050.021.23404710-11
GAGTACG82750.020.18375212-13
AGTACGG78250.018.46596312-13
Produced by FastQC (version 0.11.5)