FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111330
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18250.16421764912312276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT22400.035.6055341
TGGTATC22550.035.1057972
GTATCAA79900.025.7811661
AGAGTAC57150.024.59188311
CAGAGTA58550.024.56755310
TAACGGC801.6077296E-524.06334736
TATCAAC86300.023.6652412
GGTATCA41600.022.4116541
AACGCAG93050.021.9731226
TCAACGC93400.021.8319364
ATCAACG93450.021.7928073
CAACGCA94450.021.6765165
CGTTGCG650.003234794121.15935552
CGGGATA2400.020.62665417
CGGAACA956.0577222E-520.26387235
AACGGCC1108.248324E-620.00070437
ACGCAGA103500.019.8085757
CGCAGAG103350.019.7637758
CGAGTGT700.004974916619.64354935
CGGACAG700.004995682319.6294065
Produced by FastQC (version 0.11.5)