FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930234
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30560.32851949079478926No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28440.30572952611923454No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17770.19102720390783395No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA17380.18683471040619887No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT14960.16081975072938637No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC13960.15006976739186054No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA13250.14243727922221722No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT13220.14211477972209144No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT13090.14071728188821306No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA12590.13534229021945016No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11800.12684980338280477No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG11600.1246998067152996No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA11380.12233481038104392No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT10470.11255232554389541No Hit
GTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGA10140.1090048310425119No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC10130.10889733120913662No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC22700.037.6303752
GTATCAA72800.037.493741
GTGGTAT24750.034.356581
AACGCAG82100.034.1640366
TATCAAC82350.033.802282
TCAACGC85700.032.580144
ATCAACG86750.032.1857953
CAACGCA87550.031.9402335
ACGCAGA91650.030.6504827
CGCAGAG92450.030.3852548
TTGTACG1551.3460522E-1030.159863
GCAGAGT98700.028.4196329
GTCGAGG9350.026.8169128
GGTATCA40950.026.5791571
GCTTGTA5500.026.2827841
GCTTGGG1751.6134436E-824.295011
CAGAGTA59150.023.89068210-11
GACAGGT4500.023.620151
AAGTACG1452.0706284E-623.457251
GCTTGGC2053.4524419E-922.8136061
Produced by FastQC (version 0.11.5)