FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749456
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9780.1304946521210051No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA9200.12275570547170214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAT558.6835644E-735.0056536
TGGTATC19500.032.421652
GTGGTAT19950.032.256541
GGCTCCG400.00786521127.50444249
GGCCGAC705.705173E-627.49893255
CGGGTCG606.3366766E-527.4860866
GCGCCAG759.739353E-625.66909815
GTATCAA66950.025.3853241
TCCGCGC801.6052512E-524.06638747
TTGTACG1502.3646862E-1123.8212763
GCTTGGC1052.0959124E-723.5720881
GCTTGTA3500.023.5720861
CAGAGTA49550.023.53558310
AGAGTAC49150.023.44732511
CGGGTCC600.00203296222.92036617
CGGGCCA600.002040868422.9050714
CCGCGCA852.5655734E-522.65071548
TCTATCG852.5748559E-522.64013331
TATCAAC74650.022.572132
AACGCAG75550.022.047086
Produced by FastQC (version 0.11.5)