FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293284
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT78070.6036570467120911No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52510.40602064202448956No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36040.2786704235109999No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA22970.17760986759288755No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC16360.12649967060599218No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT14970.11575183795670557No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG13490.1043081024740119No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT13120.10144716860333847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC20800.056.3982852
GTGGTAT22050.052.2413561
GTATCAA75050.040.32581
GGTATCA39700.038.3306581
TATCAAC85700.035.3117562
TCAACGC91500.032.8850674
ATCAACG91750.032.8417853
CAACGCA92900.032.4822435
AACGCAG94950.031.82576
ACGCAGA107650.028.0305047
CGCAGAG108700.027.7597378
GCAGAGT112200.026.8937939
CAGAGTA60900.026.1698110-11
CGTCAGA2500.025.5008475
AGCGTCA2600.024.5190963
GAGTACG68350.023.1610112-13
AGAGTAC59150.022.20190810-11
ACGGGTA4950.021.463716-17
TATACCG1000.00315526521.2507065
GTACGGG79050.019.81095314-15
Produced by FastQC (version 0.11.5)