FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293284
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26270.20312630481781266No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT19270.1490005288861534No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT14330.110803195585811No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC14240.11010729275240395No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12700.032.064111
TGGTATC13350.030.484062
GTATCAA61500.029.1250761
GGTATCA29700.027.7923971
CGGCTCG400.00786962727.50276847
TATCAAC73150.024.997682
AGAGTAC69000.024.22869711
TCAACGC74700.024.1760864
ATCAACG74900.024.1143283
AACGCAG75300.024.092966
CAACGCA76500.023.8946935
CAGAGTA70650.023.85559710
ACGCAGA78150.023.2143337
CGCAGAG77800.023.0378958
GCAGAGT81450.022.0080599
TGCCGAC5400.021.9003555
AGTACGG76700.021.29857813
GAGTACG77100.021.29344612
ACGGGTC4850.020.98067916
ACGGGTA3700.020.812116
Produced by FastQC (version 0.11.5)