FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037122
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43090.41547667487527984No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36780.3546352309564352No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26080.2514651121083151No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA19230.18541695191115415No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT18300.17644982943183155No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC16470.1588048464886484No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16150.15571938499038684No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT15790.15224824080484264No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT14370.13855650540630707No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG14230.13720661600081765No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA14050.13547104390804554No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT14050.13547104390804554No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA13890.13392831315891476No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC12090.11657259223119362No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG11480.11069093125013257No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA11080.10683410437730566No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA10650.10268801548901672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC19800.054.517822
GTGGTAT19950.053.065821
GTATCAA80250.042.013281
TATCAAC90950.037.4282722
GGTATCA40350.035.720261
TCAACGC98250.034.8636174
ATCAACG98550.034.671243
AACGCAG100050.034.363826
CAACGCA100500.033.956235
ACGCAGA113150.030.4604477
CGCAGAG114150.030.156378
GCAGAGT119050.028.915169
GTCGAGG15000.026.6321728
GTACGGA1851.0513759E-925.2801361
CAGAGTA63700.025.18532210-11
TCGAGGT16150.023.420039
GACAGGT4050.023.0954321
AGAGTAC62300.022.51105710-11
AGTCGAG22100.022.499027
GAGTACG71950.022.09076512-13
Produced by FastQC (version 0.11.5)