FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690866
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24060.1422939487812754No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23980.14182081844451305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT19350.047.2413521
TGGTATC20100.045.247012
GTATCAA62300.037.5352631
TATCAAC70800.032.8942072
TCAACGC73100.031.9173724
ATCAACG73950.031.4930383
AACGCAG74850.031.114366
CAACGCA74900.031.0368525
GGTATCA36100.030.3862671
ACGCAGA82550.028.2635967
CGCAGAG84350.027.8116138
GCAGAGT92700.025.3523129
TATATCG1152.7251258E-422.1730585
CAGAGTA58600.021.72057710-11
GTCGAGG9400.020.797078
AGTACGG70600.019.563692
AGAGTAC56100.019.2795610-11
GAGTACG72150.019.0929721
ATATCGC1358.035871E-418.888166
TACGGGT17600.018.593034
Produced by FastQC (version 0.11.5)