FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725359
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22580.31129413159552716No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20100.27710416497210344No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12710.17522357894504653No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT11410.15730141902147765No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA10420.1436530049258367No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA8780.12104351086841138No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT8750.12062992256248285No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8720.12021633425655434No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT8440.1163561767345549No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT8340.11497754904812651No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC8290.11428823520491231No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG7620.10505142970584222No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA7330.10105340941519991No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA7320.1009155466465571No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57300.037.4741171
TGGTATC18700.034.0929572
AACGCAG64500.033.8020256
GTGGTAT19250.033.1325761
TATCAAC67400.032.0976222
TCAACGC69050.031.392184
ATCAACG69200.031.3241353
CAACGCA70100.031.0432325
ACGCAGA71500.030.4332967
CGCAGAG72100.030.121098
GTCGAGG7750.027.9674938
GCAGAGT78250.027.6994329
GGTATCA32300.025.406771
CAGGTAT4050.025.1971191
GCTTGGC1701.1834345E-825.0118471
GCTTGTA3250.024.8579251
GACAGGT3600.024.8034131
GTCTTAC1051.465178E-424.297221
AAGTACG900.001705749123.62231
GGTACGG1452.075396E-623.4496823
Produced by FastQC (version 0.11.5)