FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725359
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13690.1887341302720446No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA10430.14379086769447957No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT7890.10877372445919882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGT2250.031.7640291
TGGTATC21050.029.1123812
GTGGTAT21750.028.5623511
GCTTGTA2950.026.0904051
GTATCAA68300.025.5965331
CTGCTTA1251.7444108E-924.19787618
AGAGTAC48350.023.48973311
AACGCAG73900.023.2817046
TATCAAC75350.023.1587662
GCTTCTA1900.023.1478771
CAGAGTA49700.023.07300810
GCAGAGC600.00202850822.92889252
AGCGGGA600.00203258322.92097934
TCAACGC78600.021.8515594
ACAGGTA3150.021.8159541
CAGGTAT2400.021.7614151
ATCAACG79500.021.742453
CAACGCA79500.021.6041835
GGCACCG2050.021.460059
CTTGAGT2200.021.234952
Produced by FastQC (version 0.11.5)