FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106122
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT69450.6278692585447175No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44500.40230643636054614No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29390.2657030598794708No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT13360.12078233684891901No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC18750.054.1698462
GTGGTAT18850.053.899531
GTATCAA59500.037.7237971
TATCAAC67000.033.1732372
ATCAACG69100.032.1636243
TCAACGC70250.031.7580854
GGTATCA33900.031.7262881
CAACGCA70800.031.4513535
AACGCAG71750.031.0349276
ACGCAGA79850.027.9399557
CGCAGAG80450.027.7315798
GCAGAGT83900.026.6418959
CAGAGTA44400.024.54967310-11
ATTCCGT900.001712115823.6085246
AGAGTAC42250.021.27281810-11
GAGTACG52300.020.84140612-13
ATACTCG1050.00418921320.2358786
CAATCCG1601.1737921E-418.5917134
GCTTGTA4150.018.4383831
TACCGGT1651.4858735E-418.0283287
Produced by FastQC (version 0.11.5)