FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472713
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16510.3492605449818389No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15160.32070199042548014No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9810.20752549644287338No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT7580.16035099521274007No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6000.1269269091393721No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.10344543094858827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC7300.051.812682
GTGGTAT7600.048.1000671
GTATCAA28200.039.1908841
GGTATCA15000.038.2563321
TATCAAC30100.036.568082
TCAACGC32050.034.3431934
ATCAACG32350.034.024713
CAACGCA33550.032.93445
AACGCAG34100.032.40326
CGCAGAG37650.029.57688
ACGCAGA37950.029.2279037
GCAGAGT39350.028.1939959
CAGAGTA20500.026.22995210-11
GTCGAGG4950.022.3245648
GAGTACG24000.022.22763612-13
ACGGGAT5200.021.65765816-17
GACAGGT2951.8189894E-1221.6137471
TCGAGGT5400.021.253529
GTACGGG25850.020.71799914-15
ACGGGCT1050.004184181320.2371643
Produced by FastQC (version 0.11.5)