FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2314773
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55650.2404123428085605No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49720.21479428004387474No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31980.13815609565171186No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT27340.11811093355590374No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA25710.11106920635414357No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA142500.037.304561
TGGTATC46500.035.3691442
GTGGTAT47700.034.6815151
AACGCAG157800.033.8797456
TATCAAC160200.033.4517672
TCAACGC166650.032.208064
ATCAACG167100.032.1721883
CAACGCA171000.031.4135845
ACGCAGA175600.030.3728647
CGCAGAG178400.029.8961588
TTGTACG2700.028.3318733
GCAGAGT192900.027.6274769
GGTATCA81500.026.6642951
GGTACGG4850.025.411063
GCTTGTA11000.023.9698771
CAGAGTA116800.023.52348310-11
GTCGAGG19800.022.1074458
GACAGGT12700.021.096171
AGAGTAC112700.020.4574610-11
AGTACGG149450.019.365012
Produced by FastQC (version 0.11.5)