FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2314773
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33600.14515462207309313No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA23320.10074421984358724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC50550.032.5722732
GTGGTAT52650.031.442631
GTATCAA162250.024.3044171
AGAGTAC117250.023.01137711
CAGAGTA120450.022.69687510
TATCAAC177550.022.0151332
AACGCAG181850.021.3411056
ATCAACG189400.020.5339243
TCAACGC188900.020.4718744
CAACGCA193000.020.0645945
GCTTGTA8650.019.7104421
GGTATCA89750.019.3643681
TAGCGCG1008.994994E-519.24947229
TTGTACG2157.2759576E-1219.1755713
ACGCAGA204650.018.9512967
CGCAGAG207500.018.564158
GCAGAGT218400.017.7646879
AGTACGG155600.017.3045213
GAGTACG158000.017.0764812
GACAGGT9600.016.6141261
Produced by FastQC (version 0.11.5)