FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410455
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30080.213264513933447No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21990.15590713634961767No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT19660.1393876444126186No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17200.12194646408428486No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12350.047.163911
TGGTATC13100.044.126612
GTATCAA52150.041.497281
GGTATCA26100.037.9552271
TATCAAC56950.037.8396572
TCAACGC59850.036.00364
ATCAACG59950.035.8726543
CAACGCA60650.035.5987745
AACGCAG61250.035.3194436
ACGCAGA67050.032.38647
CGCAGAG69500.031.183588
GCAGAGT74700.029.2403859
ACCGGTC600.006809802728.3301778
CAGAGTA51850.024.95623810-11
AGAGTAC49100.022.0265710-11
GAGTACG58700.021.97157512-13
CATACCG1203.6313338E-421.2506475
GTACGGG63150.020.38965214-15
AGTACGG55800.019.38180212-13
ATCGGTC1100.00548348519.316038
Produced by FastQC (version 0.11.5)