FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410455
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47200.33464378516152593No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC7950.030.7731632
GGTATCA20050.030.3193471
GTGGTAT8400.029.7991621
GTATCAA41700.028.2984871
TATCAAC47150.025.3021412
TCAACGC49150.023.9327454
ATCAACG49250.023.8849963
CAACGCA50200.023.5416565
AACGCAG50600.023.3572126
AGAGTAC48750.023.35226411
CAGAGTA50650.022.69020810
ACGCAGA53200.022.2681487
CGCAGAG55150.021.481558
AGTACGG54800.020.47669813
GCAGAGT58200.020.360139
GAGTACG57750.019.57079712
GTACCGT1009.0264984E-519.2398246
TACGGGG27300.018.53683315
CGATAGG1051.3059247E-418.33534222
GGACCGT1352.3593184E-618.3236436
Produced by FastQC (version 0.11.5)