FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166690
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48450.41527740873753954No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38350.3287077115600545No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29180.2501092835286151No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT21650.18556771721708423No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18130.15539689206215876No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT14380.12325467776358758No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12590.10791212747173627No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC18000.053.595172
GTGGTAT18650.052.2076031
GTATCAA70850.040.6880531
GGTATCA35600.036.9050331
TATCAAC79500.036.8853762
TCAACGC85350.034.1580354
ATCAACG86200.033.870513
CAACGCA87450.033.4349675
AACGCAG90500.032.449036
GTCGAGG13200.029.298128
CGCAGAG101800.028.8888728
GACAGGT6800.028.7624591
ACGCAGA103000.028.5523037
GCAGAGT107300.027.4500439
AGTCGAG17200.026.9321067
TCGAGGT14350.025.767779
CAGAGTA55200.024.9088710-11
CCTATAC900.001711461923.6102053
GAGTACG60950.022.38560312-13
AGAGTAC52050.021.9251410-11
Produced by FastQC (version 0.11.5)