FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166690
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28280.24239515209695806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC22900.042.2358252
GTGGTAT23700.041.1720731
CGCTAGC501.8180273E-533.00527637
GTATCAA87700.027.1732921
AGAGTAC51400.026.27365711
GCTAGCC651.08779386E-425.38867438
CAGAGTA54200.025.17007410
TATCAAC96750.024.5662522
GGTATCA44450.023.3665083
TCAACGC103750.022.7498664
ATCAACG105000.022.5052053
CAACGCA105400.022.3676575
AGTACGG60100.022.14996313
GAGTACG61150.022.03950112
CGGGATA3500.022.00257517
AACGCAG107400.021.9274236
TACGGGG26850.019.6679515
CGCAGAG121000.019.4501468
ACGCAGA121300.019.3718997
CCATGCG1008.986068E-519.25069
Produced by FastQC (version 0.11.5)