FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339766
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43840.32722132073809906No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41150.30714318769098486No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27550.20563292395836288No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT23440.17495592513916608No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16660.1243500730724619No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT15770.11770712198995945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC22050.051.654352
GTGGTAT22950.048.346861
GTATCAA79900.039.904781
TATCAAC91950.034.942242
GGTATCA40800.033.9678341
ATCAACG96050.033.406453
TCAACGC96050.033.406454
AACGCAG99100.032.464076
CAACGCA99400.032.3233345
ACGCAGA111600.028.6755337
GCTTGTA5950.028.5794241
CGCAGAG113500.028.1955038
GCAGAGT120800.026.6695359
GTCGAGG14100.025.9214958
TCGAGGT14350.024.8794369
AGTCGAG20550.022.7489627
CAGAGTA67200.022.32639310-11
TACGGGG39150.021.9280662
AGAGTAC63750.021.26785510-11
GTATTAG5550.020.6814611
Produced by FastQC (version 0.11.5)