FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509264
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21340.4190360991548588No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15620.30671714474221623No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10840.21285620032046246No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA8310.16317666279179363No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC5980.11742436143139905No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT5390.10583901473499009No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7500.054.986671
TGGTATC7600.053.6932072
TATACGG409.315836E-442.4987755
GTATCAA25700.041.8537521
TATCAAC29250.037.0574872
ATCAACG30400.035.512333
TCAACGC30600.035.2802244
CAACGCA30750.035.104685
AACGCAG31500.034.2688526
GGTATCA13400.033.631641
ACGCAGA33950.031.7958437
CGCAGAG34550.031.1206658
GCAGAGT36800.029.2179059
TACCGAC600.006803037628.3325168
CAGAGTA22400.026.4668710-11
GTCTAGC900.001706143423.6197051
GAGTACG25300.023.18114912-13
AGAGTAC21650.022.96700510-11
ACGGGGT3950.022.59428416-17
CCTATAC950.002340386422.3699723
Produced by FastQC (version 0.11.5)