FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550451
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22720.1465380073281903No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22590.14569954161724558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC16150.042.3661082
GTGGTAT16750.040.865651
GTATCAA52500.039.6811371
TATCAAC61150.034.331732
ATCAACG62700.033.2796823
TCAACGC63200.032.949154
CAACGCA64250.032.4768265
AACGCAG64150.032.4612056
GGTATCA28200.031.6604821
ACGCAGA71700.029.1023167
CGCAGAG73350.028.5056028
GCAGAGT82750.025.3710359
GTCGAGG9900.025.3268998
AGTCGAG13350.021.6467577
CAGAGTA50050.021.5693410-11
AGAGTAC47300.019.76827410-11
TCGAGGT12800.018.9253589
AGTACGG59400.018.6732222
GCTTGTA7000.018.220931
GAGTACG60750.017.9859811
Produced by FastQC (version 0.11.5)