FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550451
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26950.1738203916150849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT18650.038.0356641
TGGTATC19150.036.887092
GTATCAA64650.025.6873051
AGAGTAC52150.022.51831211
TATCAAC73200.022.4543322
GGTATCA34350.022.1719111
CAGAGTA54100.022.01095410
TCAACGC76000.021.4455494
ATCAACG76600.021.3500183
CAACGCA78000.020.9308995
AACGCAG78150.020.9258946
CGAACTA2800.019.64355929
GCTTGTA4300.019.18241
ACGCAGA86800.018.8734197
CGCAGAG88300.018.525868
GCGAACT3050.018.03459528
AGTACGG65250.017.70292513
GAGTACG67000.017.40473212
GCAGAGT95150.017.224949
CACTCGT1801.15420335E-716.806711
Produced by FastQC (version 0.11.5)