FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322837
Sequences flagged as poor quality0
Sequence length61
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9700.3004612234657118No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8080.25028110160855166No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT6160.1908083645926582No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAG5780.1790377187249293No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA4820.14930135021698254No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT4480.13876971970375143No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC4400.13629168899475586No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4280.13257464293126253No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAATCATCAAGGAT4120.12761858151327138No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4110.12730882767464696No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT4000.12390153544977806No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA3940.12204301241803138No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA3730.11553818180691804No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC3640.11275039725929803No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT3610.1118211357434247No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAG3590.11120162806617581No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA3510.10872359735718026No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT3430.10624556664818469No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA3310.10252852058469135No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAGC150.00577850155.00325826
AGTACGC251.1851869E-555.00325413
TGCTTAA1550.040.80886519
CTGCTTA1600.039.5335918
CGGGCTA504.1103522E-738.50227717
CTTAAAC1600.036.09588621
GCTTAAA1600.036.0902920
CACTGCT1800.035.1409716
ACCACTG1900.034.73889514
GTATTAG401.8961355E-434.3823621
TTAAACG1700.033.972622
TGGTATC28700.032.6710852
GTGGTAT29000.032.2482871
CGGGCCC350.00409411731.43043317
TTACGCA350.00410033431.4206923
GACAGAC453.784798E-430.56211
GGGGGCT453.788239E-430.55736418
ACTGCTT2100.030.1208317
TAAACGC1950.029.61713823
CCACTGC2150.029.42034315
Produced by FastQC (version 0.11.5)