FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764803
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29860.3904273388048949No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23700.3098837216904223No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17550.22947085720113544No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT12730.16644809186156434No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11870.15520336609558277No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT8760.11453929966278899No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8420.11009371040647069No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC10200.058.331272
GTGGTAT10800.055.1158681
GTATCAA44150.042.2771951
GGTATCA22400.037.7726481
TATCAAC50150.037.710532
ATCAACG54550.034.5908933
TCAACGC55050.034.4311144
CAACGCA56550.033.5178225
AACGCAG57950.032.7080766
GTCGAGG8900.030.5607178
TCGAGGT8900.029.1300899
CGCAGAG65750.028.763248
ACGCAGA65900.028.7622597
GCAGAGT69200.027.3924379
CAGAGTA34400.025.08069410-11
AGTCGAG13550.024.7777217
AGAGTAC32650.022.51981710-11
GAGTACG39950.021.27723312-13
GACAGGT4700.020.806661
GCCTAAT1455.48451E-520.525911
Produced by FastQC (version 0.11.5)