FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764803
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23270.30426135880743144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT15500.045.2416651
TGGTATC15600.044.9369542
CGCCCTT350.004094965831.43455324
CGTTCGT453.794499E-430.55537410
GTATCAA57000.025.714761
AGAGTAC36750.024.39441311
CAGAGTA38050.023.99460210
GGTATCA30950.023.5320453
TATCAAC63950.022.9556062
ACACCGT852.5780899E-522.6365576
ACGGGCA1901.8189894E-1221.71039816
ATCAACG68500.021.3048463
TCAACGC68950.021.125944
CAACGCA70050.020.7941995
AACGCAG71500.020.5289366
AGTACGG43000.020.40104113
ACGGGAG5650.019.95563316
GAGTACG44650.019.95508812
TACGGGC4150.019.21675315
GTACGGG46200.018.80941214
Produced by FastQC (version 0.11.5)