FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682672
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29940.17793129023362841No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27530.16360883166772847No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18050.10726986602261165No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC22850.042.5917472
GTGGTAT23350.041.152041
GTATCAA73950.038.866761
TATCAAC86150.033.9398042
ATCAACG89100.032.482213
TCAACGC88950.032.3936544
GGTATCA41900.032.3702771
AACGCAG89450.032.2600946
CAACGCA91100.031.629155
ACGCAGA100050.028.884717
CGCAGAG101700.028.3325028
GCAGAGT110200.026.2636229
CAGAGTA67150.023.35513310-11
GTCGAGG11100.022.5894268
ACGGGTA4350.022.4706045
AGAGTAC65850.020.97633410-11
AGTCGAG16750.019.2830167
TCGAGGT13600.018.7500089
GAGTACG84900.018.44725612-13
AGTACGG82250.017.9812342
Produced by FastQC (version 0.11.5)