FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657264
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10970.16690401421650963No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAG7090.10787141848633121No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCT6670.10148129214440468No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCA150.005779250555.0078451
TGGTATC17650.042.359842
GTGGTAT17900.041.9377441
CACGTCC400.00786524327.50392321
CTAGCAC400.00789451227.4829963
CGGGGTC1700.025.88604417
ACGGATG550.001228242924.9921468
GGTATCA32750.023.7486653
AGAGTAC36000.023.53113411
GTATCAA57350.023.3502181
TAGTCGG600.002032920722.91993351
CAGAGTA37100.022.7593110
CGGGACA1103.280402E-722.50149518
CGGGATA2100.022.2650817
GACAGGT2350.022.2321341
ACGGGGT5050.021.24065216
TAGGACC650.003249928121.1407644
TATCAAC63750.020.9980472
AGTACGG41250.020.33623313
TACAGAC956.0834303E-520.2506283
Produced by FastQC (version 0.11.5)