FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743680
Sequences flagged as poor quality0
Sequence length91
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52540.706486660929432No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33890.45570675559380375No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31650.4255862736660929No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19450.2615372203098107No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT17510.23545073149741827No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16410.22065942340791736No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12280.16512478485370052No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT12200.16404905335628228No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10650.14320675559380378No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10320.1387693631669535No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9750.13110477624784853No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAA9210.12384358864027539No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8890.11954066265060241No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT8410.11308627366609293No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA7800.10488382099827884No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC13850.063.2098962
TCGACGG302.2552616E-456.663899
GTGGTAT15550.056.3070561
GCGTCAG1150.048.047581
CGTCAGA1300.039.2288442
TCCGTGC559.561339E-538.634478
CCGGTTG559.561339E-538.634479
GGTATCA31300.038.4298021
GTATCAA57950.037.1860921
TCGAGGT12750.035.6649179
GTCGAGG13900.034.5486648
GTAGGAC751.4834815E-533.9983333
TATCAAC66300.033.01122
TCAACGC70750.030.634544
ATCAACG71400.030.3556523
CAACGCA72050.030.1997665
TATAGAG600.006805692328.3319452
TCCTAGG755.9053674E-428.3319442
TACTCCG755.9053674E-428.3319445
AGTCGAG19600.028.1873937
Produced by FastQC (version 0.11.5)