FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743680
Sequences flagged as poor quality0
Sequence length61
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24760.33293889845094665No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11840.1592082616179002No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11370.15288833907056798No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGG8680.11671686746987953No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGAC150.00577312655.023349
GCTACGC150.005788523254.98627520
TGGTATC17900.044.0544932
GTGGTAT18250.042.7723271
CGGCGCT300.001921821336.6797323
TCGGCGC300.001922456736.6772622
AGATCGG300.001926272836.6624519
AACTCGG453.7863536E-430.5664424
TCGCTCT553.511238E-529.9723424
TGTCCGG653.1998388E-629.61995338
TGTACGA752.9388139E-729.33785610
GATACCA1900.028.95378311
TGATCGG606.296615E-527.5116549
TCCGGCC805.1591996E-727.50979825
CATCCGG805.1591996E-727.50979823
TCATCCG805.162219E-727.50794422
TTAAACG1500.027.50794422
CCGGGGC400.00786544927.50424240
CGGGATG805.168313E-727.50424217
GTCCGGT705.6965346E-627.50424239
Produced by FastQC (version 0.11.5)