FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391384
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56060.40290818350649427No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52670.3785439533586702No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34050.24472036475911754No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT27010.19412326144328237No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20640.14834150744869856No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT18470.1327455253186755No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAA17130.1231148266761728No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCT14500.1042127838181264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC22050.058.9772
GTGGTAT26750.048.6286661
GTATCAA86500.037.890731
TATCAAC96450.034.6330032
GGTATCA47800.034.4173771
ATCAACG105700.031.5217933
TCAACGC106050.031.4979084
CAACGCA107200.031.2392985
AACGCAG110700.030.4051676
GTCGAGG15750.028.8717488
CGCAGAG123850.027.1768448
ACGCAGA124600.027.0814747
GCAGAGT130350.025.821659
TCGAGGT17650.024.800619
CAGAGTA65800.024.67216910-11
GACAGGT5500.023.9603821
AGTCGAG26100.021.6561367
GAGTACG74650.021.43408612-13
AGAGTAC62350.020.75490210-11
GGTACGG1050.004188155320.2372063
Produced by FastQC (version 0.11.5)