FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391384
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25890.1860737222793995No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAG14730.10586581418213807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC30500.043.25492
GTGGTAT32100.041.282011
GTATCAA108800.026.2040671
GGTATCA57600.023.71273
AGAGTAC70550.023.5484211
TATCAAC121550.023.290362
CAGAGTA74000.022.89659310
CGGGGTA3050.021.64145517
ATCAACG132700.021.3103983
TCAACGC133050.021.2749944
CAACGCA136100.020.7982225
AACGCAG137900.020.4691666
CGGGATA4200.020.29959717
AGTACGG83050.019.80467813
GAGTACG83550.019.65394412
TACGGGG32000.018.82350215
GACAGGT4100.018.7755641
CGCAGAG152300.018.5204148
ACGCAGA152750.018.461877
GCAGAGT156600.017.9812859
Produced by FastQC (version 0.11.5)