FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447583
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69460.47983431692690504No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT53390.3688216841452269No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40220.27784244495825106No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT29480.2036498079902845No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27750.19169885250103103No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT19850.13712512512235914No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17360.1199240388979423No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16930.11695357019252091No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAA16200.11191068146006136No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15020.10375916268704453No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC26150.056.394672
GTGGTAT26750.054.840411
GTATCAA98250.040.8116841
GGTATCA51800.037.1034281
TATCAAC112150.036.6443862
ATCAACG120600.034.358773
TCAACGC121300.034.0203444
CAACGCA123800.033.299015
AACGCAG127750.032.369226
CGCAGAG141700.029.3335298
ACGCAGA142550.029.0681677
GTCGAGG15850.028.1549878
GCAGAGT148350.027.989969
CAGAGTA74750.025.04551710-11
AGCGTCA2401.8189894E-1224.7911743
TCGAGGT17150.024.5338849
AGAGTAC70700.022.78321610-11
GAGTACG83450.022.20523812-13
GACAGGT7750.021.9464191
CAGCGTC2759.094947E-1221.6359332
Produced by FastQC (version 0.11.5)