FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447583
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35430.24475280519320827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC31200.044.5792432
GTGGTAT31850.043.7678641
GTATCAA118850.027.0903451
GGTATCA59450.024.6885813
AGAGTAC71300.024.26263811
TATCAAC132500.024.0023772
CAGAGTA74300.023.43024310
ATCAACG142800.022.1540873
TCAACGC143000.022.0646784
CAACGCA145800.021.640945
CGATAGG650.003236939221.15746322
AACGCAG149400.021.1032626
AGTACGG81650.021.05308513
GACAGGT6100.020.7340831
GAGTACG83050.020.69818712
ACGGGGT9250.019.92163716
CGGGGTA3700.018.58297217
GTACGGG91500.018.54624414
CGGGATA4600.018.53449617
CGCAGAG170550.018.4881528
Produced by FastQC (version 0.11.5)