FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327447
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24830.18705078244178486No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23880.1798941878658809No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15630.11774481391724115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT17050.044.889921
TGGTATC17500.044.4410862
GTATCAA59050.040.5405041
TATCAAC69250.034.612342
TCAACGC70750.033.7583734
ATCAACG71500.033.5825773
GGTATCA31500.033.2066651
CAACGCA72300.033.1522035
AACGCAG72200.033.139266
ACGCAGA80400.029.8122447
CGCAGAG82100.029.2467048
GCAGAGT86950.027.5685469
AGCGTCA3200.026.561443
CAGAGTA54650.023.29310210-11
GTCGAGG9850.022.867118
TACGGGT16400.021.508294
AGAGTAC52650.020.58555210-11
AGTACGG68500.020.2254712
GAGTACG68700.020.1153371
GCAGCGT4450.019.110441
Produced by FastQC (version 0.11.5)