FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327447
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20950.15782174354230338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT20750.036.707751
TGGTATC20500.036.462732
ACCGTCC858.816096E-725.8752928
GTATCAA72050.025.5321981
GGTATCA38100.024.3220981
TATCAAC80650.022.6595572
AGAGTAC57100.022.20794111
CAGAGTA58250.022.00561110
TCAACGC84500.021.463724
ATCAACG85750.021.1516363
AACGCAG86750.020.9711696
CAACGCA86650.020.9628665
ACGCAGA95550.018.9843837
CGCAGAG97100.018.6586578
CCGTCCA909.819814E-418.332489
GCAGAGT100900.017.9600149
AGTACGG72100.017.35879713
GAGTACG72850.017.29336212
GCTTGTA4000.017.186051
ATCGGTC2303.8926373E-1016.743414
Produced by FastQC (version 0.11.5)