FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1263864
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52550.41578840761347746No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35070.27748238734547387No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25420.2011292354240646No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA22710.1796870549362906No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT20940.1656823835475969No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC15950.12620028737269198No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT15400.12184855332535778No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT15000.11868365583638746No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG14070.11132526917453144No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA13800.10918896336947646No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC13080.10349214788932985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT15900.050.5375481
TGGTATC16200.050.365072
GTATCAA62650.042.074611
GGTATCA32450.037.9955521
TATCAAC72600.036.4080162
TCAACGC74750.035.358034
ATCAACG75150.035.169833
CAACGCA75950.034.7993775
AACGCAG77900.033.9282766
ACGCAGA87050.030.362017
CGCAGAG87400.030.1918038
GCAGAGT90850.029.092059
CAGAGTA56650.025.9163710-11
GTACTGG5350.024.6352751
GAGTACG64250.022.6854312-13
AGAGTAC54700.022.56757510-11
TTCTACG1000.00315775821.2477652
AGTACGG62400.019.57849512-13
GTACGGG74250.019.37261614-15
TATATAG2001.5680016E-619.1229882
Produced by FastQC (version 0.11.5)