FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1263864
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45870.36293461954767287No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT19920.15761189495072256No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT14730.11654735003133249No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC13910.1100593101789433No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC12670.10024812796313527No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13350.032.9778631
TGGTATC12850.032.7348982
GTATCAA57000.028.4331021
GGTATCA27100.028.2266481
TGCCGAC6450.025.16061855
TATCAAC65350.024.9477732
ATCAACG68000.023.7668063
TCAACGC68000.023.6859664
AGAGTAC64300.023.64392911
CAACGCA69400.023.4476325
CAGAGTA65200.023.35879510
AACGCAG69850.023.2178696
ACGCAGA71400.022.5983457
CGCAGAG71150.022.445958
ACGGGAT9400.021.6528816
CGGGATA2450.021.33041417
GCAGAGT75150.021.2171639
AGTACGG70600.020.9192913
GAGTACG72200.020.67771312
TAGCTCG856.699383E-419.40531310
Produced by FastQC (version 0.11.5)