FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094991
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40350.36849617942065277No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31690.28940877139629456No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24230.22128035755545022No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT16970.1549784427451915No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15930.1454806477861462No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11870.10840271746525772No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13650.053.8875121
TGGTATC13900.052.8941462
GTATCAA60450.043.3270451
TATCAAC67900.038.4304052
GGTATCA28450.038.2588921
ATCAACG72400.035.983073
TCAACGC72900.035.794574
CAACGCA74850.034.9756055
AACGCAG77150.033.9886
ACGCAGA87200.030.0220667
CGCAGAG86850.029.9962528
GTCGAGG9450.029.2319568
GCAGAGT92850.028.1036559
CAGAGTA49400.024.21852310-11
TCGAGGT11100.024.1209229
GACAGGT6000.023.3851
AGTCGAG14900.022.5329067
AGAGTAC47050.021.95041810-11
GAGTACG57650.020.6794812-13
ACGGGTA2000.019.12575516-17
Produced by FastQC (version 0.11.5)