FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518378
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA10590.2042910771676267No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT9820.18943705172673223No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA8530.1645517363777012No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC7720.14892607325156546No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC6540.12616276153694794No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA6110.11786765642060426No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCA5980.11535983394357013No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT5920.11420237741570823No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC5850.11285201146653599No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT5800.11188746435998442No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG5510.10629309114198518No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA5270.10166326503053758No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC4200.046.5405432
GTATCAA21500.046.459871
GGTATCA10200.044.589461
GTGGTAT5050.041.243291
TATCAAC24350.041.010192
TCAACGC25850.038.4661034
ATCAACG25900.038.391843
CAACGCA26400.037.6647265
AACGCAG27300.036.423036
ACGCAGA28250.035.3486067
CGCAGAG28250.035.0477688
GCAGAGT30350.032.6227159
AGAGTCG2500.027.1958665
GTATATC808.59814E-426.5661491
CAGAGTA26800.026.16206610-11
CCGTCTT8000.025.23540584-85
GAGTACG29500.023.62580512-13
GTCCTAA1102.0119667E-423.1850011
GATCGAC1054.5893103E-922.2585220-21
AGAGTAC26300.022.05463210-11
Produced by FastQC (version 0.11.5)