FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518378
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT8550.16493755522032186No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT7780.15008352977942738No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT7750.14950480151549642No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT7320.14120969639915276No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC5620.1084150947763987No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTG5330.10282072155839946No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTCATCATGCGA5320.10262781213708914No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA5290.1020490838731582No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5190.10011998966005503No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAAAAGA5190.10011998966005503No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4150.032.4901851
TGGTATC4050.031.2239382
GTATCAA19250.030.5905441
GGTATCA9950.028.4851381
AGCGTCG400.0078637527.50390851
TATCAAC22600.026.2742392
CGGGGTC2900.025.60708817
TAATTAG550.001229462724.9866684
TGCCGAC3300.024.17010555
TCAACGC23800.024.136284
ATCAACG24050.023.9996683
AACGCAG24100.023.8381296
CAACGCA24550.023.398925
ACGCAGA24550.023.398927
GTATTAA1653.6379788E-1223.3479231
CGCAGAG25000.023.0921368
AGAGTAC25050.022.94294511
CGATCGT600.002033489822.91771147
GCCTAGC600.002039204422.9066566
CGTACAG600.00204034922.9044463
Produced by FastQC (version 0.11.5)