FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522652
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39170.25724853742023784No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17070.11210703430593463No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15260.10021987952598492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC35500.043.588142
GTGGTAT36650.042.6928251
GTATCAA141500.027.343511
AGAGTAC80250.026.55926511
CAGAGTA82100.026.26227410
TATCAAC154950.024.7972722
GGTATCA70150.023.5593571
TCAACGC167100.022.8925424
ATCAACG167600.022.8726843
AGTACGG91650.022.83555613
GACAGGT6400.022.7725941
CGGGATA6050.022.72643317
GAGTACG93100.022.7162212
CAACGCA171000.022.354365
AACGCAG173100.022.1473946
ACGGGGT11000.021.75062616
TACGGGG36150.021.37752515
AATCGGT650.003236601821.15792836
GTACGGG101350.020.56860714
TACGGGT7850.019.96938115
Produced by FastQC (version 0.11.5)