FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194901
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16700.139760532462522No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCTCACTAAGGAAG13680.11448647210103599No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAATCATCAAGGAT12200.10210050874507595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC24300.044.904532
GTGGTAT25150.044.166681
GTATCAA90200.026.5194231
AGAGTAC58550.024.94286211
CAGAGTA60200.024.53127110
GGTATCA46800.024.3142263
CGAATTA2400.024.06504815
TATCAAC100950.023.5785712
TCAACGC107750.022.0641174
ATCAACG107650.022.0344733
CAACGCA110150.021.5833745
AGTACGG68000.021.355213
AACGCAG111800.021.2165586
CCGAATT2600.021.15608614
CCGCGTT650.00324098221.15254218
CGCGTTC650.00324098221.15254219
GAGTACG69050.021.07029512
CTATCGG1251.1222728E-619.80209512
ACGGGAT14250.019.68629516
TACGGGG27550.019.36684215
Produced by FastQC (version 0.11.5)